Melamine Rapid Test Kit

MELAMINE RAPID TEST
Cat No.: W81120

1. INTENDED USE
Quicking Melamine Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of melamine residue in milk, milk powder, yogurt, feed and egg.
Detection Limit: Milk, 1.0 ppm; Milk Powder, 2.5 ppm; Yogurt, 1.0 ppm; Feed, 2.5 ppm; Egg, 1.0 ppm.
Assay Time: 5 - 10 min.

2. KIT COMPONENT
- 10× Foil pouches each containing one cassette and a desiccant
- 1× Assay buffer (50 mL)
- 10× Centrifugal tubes (1.5 mL)
- Products Manual

3. SAMPLE EXTRACTION
3.1 Milk (1.0 ppm)
Transfer 0.2 mL of milk sample into a centrifugal tube. (Tip: The volume of milk sample reaches the scale of the small pipette provided in the foil pouch). Add 0.6 mL of assay buffer into the tube and mix well with the milk sample. Use the mixture in the assay.
3.2 Milk powder (2.5 ppm)
Dissolve 0.5 g of milk powder with 5 mL of 60 C degree tap water. Keep it cool to room temperature. Use the liquid milk for running the assay.
3.3 Yogurt (1.0 ppm)
Transfer 0.2 mL of yogurt sample into a centrifugal tube. (Tip: The volume of yogurt sample reaches the scale of the small pipette provided in the foil pouch). Add 0.6 mL of assay buffer into the tube and mix well. Do centrifugation at 4000 rpm for 1 min. Take the supernatant for assay.
3.4 Feed (2.5 ppm)
Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 0.5 g ground portion of the sample into a sample tube. Add 4 mL of assay buffer into the tube and shake for 5 min. Use the supernatant in the assay. (If it is cloudy, do a centrifugation at 4000 rpm for 1 min. )
3.5 Egg (2.5 ppm)
Suck 0.2 mL of egg sample into a centrifugal tube. Add 0.8 mL of assay buffer into the tube and mix well with the sample. Use the mixture for assay.

4. TEST PROCEDURE
- Take out the cassette from the foil pouch and place it horizontally.
- Gradually drip 3 drops of sample extraction into the assay sample hole.
- Interpret the result in 5 - 10 min. Result after 10 min is considered as invalid.

5. INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Melamine is at or above the cut-off value in the sample.
Negative: The presence of both clear band in C zone and T zone.
Invalid: No colored band appears in C zone.

6. STORAGE
The kit can be stored at room temperature (2-30 C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

7. PRECAUTIONS
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not reuse the test kit or use the test beyond its expiration date marked on the foil pouch.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

8. LIMITATION
Quicking Melamine Rapid Test is a useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Melamine. If you want a quantitative result, please adopt other method such as ELISA/ HPLC in practice.